caco 2bbe cells (ATCC)

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ATCC caco 2bbe cells
Caco 2bbe Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caco+2bbe+cells/Caco-2/bio_rxiv__2024__12__20__629767-275-3-9
Average 99 stars, based on 15206 article reviews

caco 2bbe cells - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Expression, Localization and Functional Activity of the Major Na + /H + Exchange Isoforms Expressed in the Intestinal Cell Line Caco-2BBe
Article Snippet: Bacterial glycerol stocks containing PLKO.1 lentiviral vector harboring five individual, small hairpin RNA (shRNA) constructs against human NHE2 (SHCLNG-NM-003048) were obtained from Sigma (St. Louis, USA) and were used to generate lentiviral particles as described earlier [ ]. .. To evaluate the knockdown efficiency of individual shRNA against NHE2 lentiviral transduction was done as follows: Caco-2BBe cells (source ATCC® CRL-2102, kindly provided by Georg Lamprecht, Rostock) were plated at a density of 5×10 4 cells per well in 24 well plates and incubated at 37°C and 5% CO 2 until they reached 60–70% confluence. ..

shRNA:

Transduction:

Incubation:

Modification:

Article Title: A Clostridium difficile Cell Wall Glycopolymer Locus Influences Bacterial Shape, Polysaccharide Production and Virulence
Article Snippet: Second, a specific cytokine, Interleukin-8 (IL-8), was quantitated using the Human CXCL8/IL-8 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN). .. Caco-2BBe cells (ATCC CRL-2102) were propagated in 6-well plates using Dulbecco’s Modification of Eagle’s Medium (DMEM with 4.5g/L glucose, L-glutamine and sodium pyruvate) (Corning, Corning, NY) supplemented with 10% FBS and 0.5% HEPES. ..

other:

Article Title: Schlafen 12 Interaction with SerpinB12 and Deubiquitylases Drives Human Enterocyte Differentiation
Article Snippet: Caco-2BBE cells and rat IEC6 cells (American Tissue Culture Collection, ATCC) each tested negative for mycoplasmal contamination and were maintained as described [ 24 ].

Cell Culture:

Article Title: TRPV6 channel mediates alcohol-induced gut barrier dysfunction and systemic response.
Article Snippet: .. Caco-2bbe cells (ATCC; Rockville, MD, USA) were maintained under standard cell culture conditions in DMEM containing 10% (v/v) fetal bovine serum (Life Technologies, Carlsbad, CA, USA). .. HEK 293 cells HEK 293 cells (ATCC) were cultured in DMEM, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37 C and 5% CO2.

Article Title: The Septin Cytoskeleton is a Novel Regulator of Intestinal Epithelial Barrier Integrity and Mucosal Inflammation
Article Snippet: DLD1 (Cat# CCL-221) and Caco-2BBE (Cat# CRL-2102) human colonic epithelial cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA). .. HT-29, DLD1 and Caco-2BBE cells were cultured in DMEM (ATCC, Cat# 30-2002) medium supplemented with 10% fetal bovine serum (FBS), HEPES, non-essential amino acids, and penicillin-streptomycin antibiotic. .. The cells were seeded on collagen-coated Transwell filters (Falcon, Corning, NY, Cat# 353095), or glass coverslips for permeability measurements and immunolabeling experiments; and on 6-well plastic plates for other functional and biochemical studies.

Article Title: TRPV6 Channel Mediates Alcohol-Induced Gut Barrier Dysfunction and Systemic Response
Article Snippet: Avtar S. Meena1*, Pradeep K. Shukla1*, Francesco Giorgianni1, Briar Bell1, Rebeca Caires1, Carlos Fernández-Peña1, Sarka Beranova1, Eitaro Aihara2, Marshall H. Montrose2, Valeria Vásquez1, Jonathan H. Jaggar1, Julio F. Cordero-Morales1**, and RadhaKrishna Rao1** 1Departments of Physiology and Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA 2Department of Pharmacology and Systems Physiology, University of Cincinnati, Cincinnati, OH 45221, USA.

Article Title: Prevention and treatment of secretory diarrhea by the lysophosphatidic acid analog Rx100.
Article Snippet: Dry fecal pellets were extracted using HyClone WFI quality water (Fisher Scientific. and subjected to chloride assay (QuantiChrome Chloride Assay Kit, BioAssays Systems, Highland, UT) as per the manufacturer’s instructions. .. Caco-2bbe cells (ATCC, Rockville, MD) were grown under standard cell culture conditions as described previously.43 Experiments were conducted using cells grown in polycarbonate membrane transwell inserts (6.5 mm diameter) (Costar, Sigma–Aldrich, St Louis, MO) for nine days. ..

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